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pcmv flag wt pten  (Addgene inc)


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    Structured Review

    Addgene inc pcmv flag wt pten
    Pcmv Flag Wt Pten, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pcmv+flag+wt+pten/pCMV+Flag+WT-PTEN+(Plasmid+%2322231)/pmc12618074__jci-135-180927-s157-180-4-21
    Average 93 stars, based on 13 article reviews
    pcmv flag wt pten - by Bioz Stars, 2026-10
    93/100 stars

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    Related Articles

    Transfection:

    Article Title: Alborixin clears amyloid-β by inducing autophagy through PTEN-mediated inhibition of the AKT pathway
    Article Snippet: Flow cytometric analysis of intracellular Aβ 1-42 Cells after various treatments were washed and resuspended in PBS for analysis by flow cytometry for the intracellular fluorescence of Aβ 1-42 -HiLyte Fluor 488 or Aβ 1-42 -HiLyte Fluor 555 in FL-1 and FL-2 channels respectively at BD FACS Calibur flow cytometer. .. Transfection of Myr-AKT delta4-129 and pCMV Flag WT-PTEN into HMC3 cells Myr-AKT delta4-129 was procured from Addgene (10841; Richard Roth) [ 49 ]. pCMV Flag WT-PTEN was obtained from Addgene (22231; Hong Wu) [ 50 ]. ..

    Article Title: Ribosomal protein S6 kinase 1 promotes the survival of photoreceptors in retinitis pigmentosa
    Article Snippet: .. The 70% confluent 661W cells were transiently transfected with a mixture composed of lipofectamine 2000 (Invitrogen) and pCMV/Flag/WT-PTEN (200ng, Addgene), with overexpression of pCMV/Flag/dPDZ-PTEN (200 ng, Addgene) as the negative control. .. P70S6K1 overexpression was realized by transfecting pRK7-HA-S6K1-WT (Addgene), and pRK7-HA-S6K1-KR (Addgene) was used as the negative control.

    Article Title: Ribosomal protein S6 kinase 1 promotes the survival of photoreceptors in retinitis pigmentosa.
    Article Snippet: .. The 70% confluent 661W cells were transiently transfected with a mixture composed of lipofectamine 2000 (Invitrogen) and pCMV/Flag/WT-PTEN (200ng, Addgene), with overexpression of pCMV/Flag/dPDZPTEN (200 ng, Addgene) as the negative control. .. P70S6K1 overexpression was realized by transfecting pRK7-HA-S6K1-WT (Addgene), and pRK7-HA-S6K1KR (Addgene) was used as the negative control.

    Expressing:

    Article Title: USP10/GSK3β-mediated inhibition of PTEN drives resistance to PI3K inhibitors in breast cancer
    Article Snippet: .. The expression plasmids pCDNA3-GFP-PTEN, pCMV- FLAG WT-PTEN, MYC- PTEN, PCI-neo FLAG-HAUSP, FLAG-USP13, pRK-HA-ubiquitin and all ubiquitin mutant subvariants were purchased from Addgene. pCMV-FLAG-USP10 and pCMV-USP10 was purchased from the MRC unit at the University of Dundee. .. The catalytically inactive USP10 was made using site-directed mutagenesis kit (Agilent) and confirmed by DNA sequencing.

    Ubiquitin Proteomics:

    Article Title: USP10/GSK3β-mediated inhibition of PTEN drives resistance to PI3K inhibitors in breast cancer
    Article Snippet: .. The expression plasmids pCDNA3-GFP-PTEN, pCMV- FLAG WT-PTEN, MYC- PTEN, PCI-neo FLAG-HAUSP, FLAG-USP13, pRK-HA-ubiquitin and all ubiquitin mutant subvariants were purchased from Addgene. pCMV-FLAG-USP10 and pCMV-USP10 was purchased from the MRC unit at the University of Dundee. .. The catalytically inactive USP10 was made using site-directed mutagenesis kit (Agilent) and confirmed by DNA sequencing.

    Mutagenesis:

    Article Title: USP10/GSK3β-mediated inhibition of PTEN drives resistance to PI3K inhibitors in breast cancer
    Article Snippet: .. The expression plasmids pCDNA3-GFP-PTEN, pCMV- FLAG WT-PTEN, MYC- PTEN, PCI-neo FLAG-HAUSP, FLAG-USP13, pRK-HA-ubiquitin and all ubiquitin mutant subvariants were purchased from Addgene. pCMV-FLAG-USP10 and pCMV-USP10 was purchased from the MRC unit at the University of Dundee. .. The catalytically inactive USP10 was made using site-directed mutagenesis kit (Agilent) and confirmed by DNA sequencing.

    Over Expression:

    Article Title: Ribosomal protein S6 kinase 1 promotes the survival of photoreceptors in retinitis pigmentosa
    Article Snippet: .. The 70% confluent 661W cells were transiently transfected with a mixture composed of lipofectamine 2000 (Invitrogen) and pCMV/Flag/WT-PTEN (200ng, Addgene), with overexpression of pCMV/Flag/dPDZ-PTEN (200 ng, Addgene) as the negative control. .. P70S6K1 overexpression was realized by transfecting pRK7-HA-S6K1-WT (Addgene), and pRK7-HA-S6K1-KR (Addgene) was used as the negative control.

    Article Title: Ribosomal protein S6 kinase 1 promotes the survival of photoreceptors in retinitis pigmentosa.
    Article Snippet: .. The 70% confluent 661W cells were transiently transfected with a mixture composed of lipofectamine 2000 (Invitrogen) and pCMV/Flag/WT-PTEN (200ng, Addgene), with overexpression of pCMV/Flag/dPDZPTEN (200 ng, Addgene) as the negative control. .. P70S6K1 overexpression was realized by transfecting pRK7-HA-S6K1-WT (Addgene), and pRK7-HA-S6K1KR (Addgene) was used as the negative control.

    Article Title: Interleukin-like EMT inducer regulates partial phenotype switching in MITF-low melanoma cell lines
    Article Snippet: MITF coding sequence overexpression vector was generated as follows: ApaI/EcoRI double digest was conducted on pCMV-Tag4A-MITF-M (wt), and ligated into the ApaI/EcoRI sites of pLenti-puro. pCMV-Tag4A-MITF-M (wt) was a generous gift from Yarden Samuels (Addgene plasmid #31151). pLenti-puro was a gift from Ie-Ming Shih (Addgene plasmid #39481) [ ]. .. PTEN coding sequence overexpression vector was generated as follows: PTEN coding sequence was amplified from pCMV Flag WT-PTEN with BamHI and EcoRI sites by PCR, the PCR product was double digested by BamHI and EcoRI, and ligated into the BamHI/EcoRI sites of pLenti-puro. pCMV Flag WT-PTEN was a gift from Hong Wu (Addgene plasmid #22231) [ ]. ..

    Negative Control:

    Article Title: Ribosomal protein S6 kinase 1 promotes the survival of photoreceptors in retinitis pigmentosa
    Article Snippet: .. The 70% confluent 661W cells were transiently transfected with a mixture composed of lipofectamine 2000 (Invitrogen) and pCMV/Flag/WT-PTEN (200ng, Addgene), with overexpression of pCMV/Flag/dPDZ-PTEN (200 ng, Addgene) as the negative control. .. P70S6K1 overexpression was realized by transfecting pRK7-HA-S6K1-WT (Addgene), and pRK7-HA-S6K1-KR (Addgene) was used as the negative control.

    Article Title: Ribosomal protein S6 kinase 1 promotes the survival of photoreceptors in retinitis pigmentosa.
    Article Snippet: .. The 70% confluent 661W cells were transiently transfected with a mixture composed of lipofectamine 2000 (Invitrogen) and pCMV/Flag/WT-PTEN (200ng, Addgene), with overexpression of pCMV/Flag/dPDZPTEN (200 ng, Addgene) as the negative control. .. P70S6K1 overexpression was realized by transfecting pRK7-HA-S6K1-WT (Addgene), and pRK7-HA-S6K1KR (Addgene) was used as the negative control.

    Sequencing:

    Article Title: Interleukin-like EMT inducer regulates partial phenotype switching in MITF-low melanoma cell lines
    Article Snippet: MITF coding sequence overexpression vector was generated as follows: ApaI/EcoRI double digest was conducted on pCMV-Tag4A-MITF-M (wt), and ligated into the ApaI/EcoRI sites of pLenti-puro. pCMV-Tag4A-MITF-M (wt) was a generous gift from Yarden Samuels (Addgene plasmid #31151). pLenti-puro was a gift from Ie-Ming Shih (Addgene plasmid #39481) [ ]. .. PTEN coding sequence overexpression vector was generated as follows: PTEN coding sequence was amplified from pCMV Flag WT-PTEN with BamHI and EcoRI sites by PCR, the PCR product was double digested by BamHI and EcoRI, and ligated into the BamHI/EcoRI sites of pLenti-puro. pCMV Flag WT-PTEN was a gift from Hong Wu (Addgene plasmid #22231) [ ]. ..

    Plasmid Preparation:

    Article Title: Interleukin-like EMT inducer regulates partial phenotype switching in MITF-low melanoma cell lines
    Article Snippet: MITF coding sequence overexpression vector was generated as follows: ApaI/EcoRI double digest was conducted on pCMV-Tag4A-MITF-M (wt), and ligated into the ApaI/EcoRI sites of pLenti-puro. pCMV-Tag4A-MITF-M (wt) was a generous gift from Yarden Samuels (Addgene plasmid #31151). pLenti-puro was a gift from Ie-Ming Shih (Addgene plasmid #39481) [ ]. .. PTEN coding sequence overexpression vector was generated as follows: PTEN coding sequence was amplified from pCMV Flag WT-PTEN with BamHI and EcoRI sites by PCR, the PCR product was double digested by BamHI and EcoRI, and ligated into the BamHI/EcoRI sites of pLenti-puro. pCMV Flag WT-PTEN was a gift from Hong Wu (Addgene plasmid #22231) [ ]. ..

    Generated:

    Article Title: Interleukin-like EMT inducer regulates partial phenotype switching in MITF-low melanoma cell lines
    Article Snippet: MITF coding sequence overexpression vector was generated as follows: ApaI/EcoRI double digest was conducted on pCMV-Tag4A-MITF-M (wt), and ligated into the ApaI/EcoRI sites of pLenti-puro. pCMV-Tag4A-MITF-M (wt) was a generous gift from Yarden Samuels (Addgene plasmid #31151). pLenti-puro was a gift from Ie-Ming Shih (Addgene plasmid #39481) [ ]. .. PTEN coding sequence overexpression vector was generated as follows: PTEN coding sequence was amplified from pCMV Flag WT-PTEN with BamHI and EcoRI sites by PCR, the PCR product was double digested by BamHI and EcoRI, and ligated into the BamHI/EcoRI sites of pLenti-puro. pCMV Flag WT-PTEN was a gift from Hong Wu (Addgene plasmid #22231) [ ]. ..

    Amplification:

    Article Title: Interleukin-like EMT inducer regulates partial phenotype switching in MITF-low melanoma cell lines
    Article Snippet: MITF coding sequence overexpression vector was generated as follows: ApaI/EcoRI double digest was conducted on pCMV-Tag4A-MITF-M (wt), and ligated into the ApaI/EcoRI sites of pLenti-puro. pCMV-Tag4A-MITF-M (wt) was a generous gift from Yarden Samuels (Addgene plasmid #31151). pLenti-puro was a gift from Ie-Ming Shih (Addgene plasmid #39481) [ ]. .. PTEN coding sequence overexpression vector was generated as follows: PTEN coding sequence was amplified from pCMV Flag WT-PTEN with BamHI and EcoRI sites by PCR, the PCR product was double digested by BamHI and EcoRI, and ligated into the BamHI/EcoRI sites of pLenti-puro. pCMV Flag WT-PTEN was a gift from Hong Wu (Addgene plasmid #22231) [ ]. ..

    Polymerase Chain Reaction:

    Article Title: Interleukin-like EMT inducer regulates partial phenotype switching in MITF-low melanoma cell lines
    Article Snippet: MITF coding sequence overexpression vector was generated as follows: ApaI/EcoRI double digest was conducted on pCMV-Tag4A-MITF-M (wt), and ligated into the ApaI/EcoRI sites of pLenti-puro. pCMV-Tag4A-MITF-M (wt) was a generous gift from Yarden Samuels (Addgene plasmid #31151). pLenti-puro was a gift from Ie-Ming Shih (Addgene plasmid #39481) [ ]. .. PTEN coding sequence overexpression vector was generated as follows: PTEN coding sequence was amplified from pCMV Flag WT-PTEN with BamHI and EcoRI sites by PCR, the PCR product was double digested by BamHI and EcoRI, and ligated into the BamHI/EcoRI sites of pLenti-puro. pCMV Flag WT-PTEN was a gift from Hong Wu (Addgene plasmid #22231) [ ]. ..



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